Professor Guo Xuefeng from the School of Chemistry and Molecular Engineering at Peking University has developed a mature experimental technology for preparing single molecule chips, which mainly reveals two key steps: the preparation of graphene field-effect transistors and single molecule anchoring. The single molecule devices produced by these technologies have universality, which will lead to the birth of a new generation of single molecule electronic devices, and cross integrate with other disciplines, promoting the development of new fields of single molecule cross science, such as the development of single molecular physics and basic physical properties of chemistry, single molecule chemical reaction dynamics and single molecule biophysics. The relevant research results are titled "Graphene Molecule Graphene Monomolecular Junction for Detecting Electronic Reactions at the Molecular Scale" and were recently published in the international academic journal "Nature Protocol". Guo Xuefeng introduced that single molecules are the basic units of the material world, the genes that construct the material world, and the key to regulating life processes, with rich scientific connotations. Among them, graphene based single molecule devices have certain interface coupling, high stability, and good resistance to complex environments, which are expected to create a strong engine for exploring infinite bottom space. During the research process, the team grew high-quality single-layer graphene on copper sheets through chemical vapor deposition. With the assistance of polymethyl methacrylate, graphene is transferred to specific size silicon wafers after etching copper sheets to meet subsequent testing requirements. Afterwards, graphene was cut into stripe patterns through oxygen plasma etching. Furthermore, graphene field-effect transistors were obtained by evaporating and preparing electrode arrays. Anchoring a single molecule requires the preparation of a series of spaced graphene electrode pairs. By Electron beam lithography, dotted line windows are prepared on the spin coated polymethyl methacrylate (PMMA) on the graphene surface. In combination with further oxygen plasma isotropic etching and assisted electrical burn off, graphene point contact can be obtained, and then electrode pairs with carboxyl end and nano gap can be prepared. Subsequently, the research team added graphene chips into the flask, and prepared covalent bond anchored single molecular junctions using click chemistry or covalent condensation according to the functional groups at the ends of different molecular bridges. Among them, anchored individual molecules can be further validated through multimodal characterization such as electrical and optical properties, including gate voltage dependence experiments, stochastic optical reconstruction super-resolution imaging, single molecule spectroscopy, and inelastic electron tunneling spectroscopy. These technologies utilize individual molecules to prepare optoelectronic devices, taking a crucial step towards the potential application of molecular electronic devices. They are expected to develop disruptive single molecule chip integration technology and a new generation of precise molecular diagnosis/sequencing technology. They are also expected to provide epoch-making research paradigms and spectroscopic methods for revealing the intrinsic mechanisms of material conversion and the intrinsic laws of life phenomena, thereby promoting the formation of new growth points in single molecule interdisciplinary science A technological breakthrough Guo Xuefeng said. (Outlook New Era Network)